Ligases
A Ligase is an enzyme that can ligate ("glue together") two molecules with a covalent bond, using energy from ATP.
DNA ligase can ligate broken DNA strands, forming covalent phosphodiester bonds.
An example of how a ligase works (with sticky ends):
5'-AGTCTGATCTGACT GATGCGTATGCTAGTGCT-3'
3'-TCAGACTAGACTGACTACG CATACGATCACGA-5'
becomes
5'-AGTCTGATCTGACTGATGCGTATGCTAGTGCT-3'
3'-TCAGACTAGACTGACTACGCATACGATCACGA-5'
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